Monday, March 4, 2019
DNA sequencing
tie the limitations of traditional methods of aiming bacteria. Traditional methods include judging by phenotypes characteristics. This could be a problem if two bacteria looked similar because they could easily be confused. iterate the goal of each of the six parts of the lab. Sample Prep first base we need to extract the deoxyribonucleic acid from the bacterial wall PC involution Create millions of copies of the initial DNA PC purification Filter come forward DNA from primers and enzymes Sequencing Prep Run more PC to make copies of DNA at various lengths DNA SequencingSequence machine runs gel electrophoresis to Identify nucleotides and trammel instalment. Sequence Analysis Computer determines full DNA sequence which after part be run and comp ared. Explain how fluorescent markers help determine a nucleotide sequence. Each nucleotide is assigned a certain(a) color. Conclusion 1 How can scientists identify specific bacteria when they are amplifying and studying the same re gion of DNA In each species? They can identify it because there are unique sequences for each species. If you take a sample and find the DNA of it you can then compare it to the Donas of know bacteria to discover what bacteria the sample is from
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